Strategy Model in Bus Tracking and Information Application (BTA) Towards Smart Mobility in Urban Spaces

Smart city can be defined as an urban space with complete and advanced infrastructure, intelligent networks and platforms, with millions of sensors among which people themselves and their mobile devices. Urban mobility is one of the global smart city project which offers traffic management in real-time, management of passenger transport means, tracking applications and logistics, car sharing services, car park management and more smart mobility services. Due to the frustrating waiting time for the arrival of buses and the difficulty of accessing shuttle bus-related information in a onestop centre, bus tracking and information application (BTA) is one the proposed solutions to solve the traffic problems in urban spaces. This paper is aimed to design and develop a bus tracking and information application in a selected city in Selangor state, Malaysia. Next, this application also provides an alternative to design public transport tracking and information application for the urban places in Malaysia. Furthermore, the application also provides a smart solution for the management of public infrastructures and urban facilities in Malaysia in future. Finally, the smart mobility model will be presented to uncover the extent on how BTA provide smart solutions in urban spaces which focus on informative, interactive, assistive and green mobility

Strategy model in bus tracking and information application (BTA) towards smart mobility in urban spaces

Smart city can be defined as an urban space with complete and advanced infrastructure, intelligent networks and platforms, with millions of sensors among which people themselves and their mobile devices. Urban mobility is one of the global smart city project which offers traffic management in real-Time, management of passenger transport means, tracking applications and logistics, car sharing services, car park management and more smart mobility services. Due to the frustrating waiting time for the arrival of buses and the difficulty of accessing shuttle bus-related information in a one-stop centre, bus tracking and information application (BTA) is one the proposed solutions to solve the traffic problems in urban spaces. This paper is aimed to design and develop a bus tracking and information application in a selected city in Selangor state, Malaysia. Next, this application also provides an alternative to design public transport tracking and information application for the urban places in Malaysia. Furthermore, the application also provides a smart solution for the management of public infrastructures and urban facilities in Malaysia in future. Finally, the smart mobility model will be presented to uncover the extent on how BTA provide smart solutions in urban spaces which focus on informative, interactive, assistive and green mobility

Molecular cloning and characterization of two pathogenesis-related beta-1,3-glucanase genes ScGluA1 and ScGluD1 from sugarcane infected by Sporisorium scitamineum

Key message: Two β-1,3-glucanase genes from sugarcane were cloned and characterized. They were all located in apoplast and involves in different expression patterns in biotic and abiotic stress. Smut caused by Sporisorium scitamineum is a serious disease in the sugarcane industry. β-1,3-Glucanase, a typical pathogenesis-related protein, has been shown to express during plant-pathogen interaction and involves in sugarcane defense response. In this study, β-1,3-glucanase enzyme activity in the resistant variety increased faster and lasted longer than that of the susceptible one when inoculated with S. scitamineum, along with a positive correlation between the activity of the β-1,3-glucanase and smut resistance. Furthermore, two β-1,3-glucanase genes from S. scitamineum infected sugarcane, ScGluA1 (GenBank Accession No. KC848050) and ScGluD1 (GenBank Accession No. KC848051) were cloned and characterized. Phylogenetic analysis suggested that ScGluA1 and ScGluD1 clustered within subfamily A and subfamily D, respectively. Subcellular localization analysis demonstrated that both gene products were targeted to apoplast. Escherichia coli Rosetta (DE3) cells expressing ScGluA1 and ScGluD1 showed varying degrees of tolerance to NaCl, CdCl, PEG, CuCl and ZnSO. Q-PCR analysis showed up-regulation of ScGluA1 and slight down-regulation of ScGluD1 in response to S. scitamineum infection. It suggested that ScGluA1 may be involved in the defense reaction of the sugarcane to the smut, while it is likely that ScGluD1 was inhibited. The gene expression patterns of ScGluA1 and ScGluD1, in response to abiotic stresses, were similar to sugarcane response against smut infection. Together, β-1,3-glucanase may function in sugarcane defense mechanism for S. scitamineum. The positive responses of ScGluA1 and the negative responses of ScGluD1 to biotic and abiotic stresses indicate they play different roles in interaction between sugarcane and biotic or abiotic stresses